Species DNA-based identification for detection of processed meat adulteration: is there a role of human short tandem repeats (STRs)?
نویسندگان
چکیده
منابع مشابه
Identification of Pork Adulteration in Processed Meat Products Using the Developed Mitochondrial DNA-Based Primers
The identification of pork in commercially processed meats is one of the most crucial issues in the food industry because of religious food ethics, medical purposes, and intentional adulteration to decrease production cost. This study therefore aimed to develop a method for the detection of pork adulteration in meat products using primers specific for pig mitochondrial DNA. Mitochondrial DNA se...
متن کاملCurrent Development for the Detection of Meat Species Adulteration
Meat species adulteration imposes substantial concern to consumers in terms of economic loss, food allergy, religious observance, and food safety. Federal and state laws require that meat products be labeled as to the species they contain. Species substitutions, such as substitution of horse meat for beef and pork for sheep meat, have been reported in several countries (Matin, 1981). As mechani...
متن کاملMutation of human short tandem repeats.
A total of 20,000 parent-offspring transfers of alleles were examined through the genotyping within 40 CEPH reference families of 28 short tandem repeat polymorphisms (STRPs) located on chromosome 19. Forty-seven initial mutation events were detected in the STRPs using DNA from transformed lymphoblastoid cell lines, but less than half (39%) could be verified using DNA from untransformed cells. ...
متن کاملOptimization of DNA concentration to amplify short tandem repeats of human genomic DNA.
Analysis of the length polymorphisms of short tandem repeats (STR) loci in the human genome has become a standard approach for comparative genotyping in many areas including disease research and diagnostics, parentage assessment, investigations of human diversity, and forensic science. The purpose of this study is to optimize the DNA concentration in ng/10 µL for amplification of DNA markers. A...
متن کاملAnalysis of Short Tandem Repeats by Parallel DNA Threading
The majority of studies employing short tandem repeats (STRs) require investigation of several of these genetic markers. As such, we demonstrate the feasibility of the trinucleotide threading (TnT) approach for scalable analysis of STRs. The TnT method represents a parallel amplification alternative that addresses the obstacles associated with multiplex PCR. In this study, analysis of the STR f...
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ژورنال
عنوان ژورنال: Egyptian Journal of Forensic Sciences
سال: 2019
ISSN: 2090-5939
DOI: 10.1186/s41935-019-0121-y